Detection of mutations in GC-rich DNA by bisulphite denaturing gradient gel electrophoresis
نویسندگان
چکیده
منابع مشابه
Detection of mutations in GC-rich DNA by bisulphite denaturing gradient gel electrophoresis.
Denaturing gradient gel electrophoresis (DGGE) in combination with PCR and 'GC-clamping' has proven highly efficient as a method for detection of DNA sequence differences. Due to strand dissociation phenomena, however, its use has been limited to the analysis of sequences with a relatively low content of GC pairs. This paper describes how treatment of template DNA with sodium bisulphite drastic...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1998
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/26.6.1548